|
چکیده
|
Background: Trypanosomiasis causes by vector born haemoprotozoa in all mammals such as equines and ruminants. In equines, most common forms are Surra, Malde- caderus, Nagana, Dourine etc. It is a vector borne disease transmitted mechanically by various species of biting flies from the blood of infected animals. Methods: A total of 118 horses blood samples (equestrian horses = 100 and farm horses = 18) from Mazandaran (Amol and Mahmoodabad Hamedan, Amol, Hamadan (Malair and Hamadan), Arak (Komijan) and Lorestan (Borujerd and Rumshkan) were sampled during 2023. Blood samples (up to 5 mL) were collected from the jugular vein into the labeled vacuum tubes containing EDTA for PCR analysis. Giemsa-stained thin blood smears were prepared immediately after peripheral blood collection and further examined microscopically. The DNA extracted from each blood sample was evaluated by molecular methods to determine Trypanosoma species. Results: Two positive cases (from equestrian horses) were showed via microscopic observation, which confirmed positive by PCR. Conclusion: Further studies are necessary to distinguish Trypanosoma species prevalence and its vectors of other regions in Iran
|