مشخصات پژوهش

عنوان	Molecular identification of haemotropic Mycoplasma species in the blood and ectoparasites of dogs in Hamedan and Kermanshah in 2019–2021
نوع پژوهش	مقاله ارائه شده کنفرانسی
کلیدواژه‌ها	Canis familiaris, flea-borne, Haemobartonella, One Health, tick-borne, zoonotic
چکیده	Haemotropic Mycoplasma species (syn. Haemobartonella) are vector-borne pathogens of different mammalian species. Infection of dogs and their ticks and fleas with these bacteria have been reported in many countries however, there is scarce information about the epidemiology of these zoonotic pathogens in dogs of Iran. The aim of this study was molecular identification of haemotropic Mycoplasma spp. in the blood and ectoparasites of dogs in Hamedan and Kermanshah. Between October 2019 to September 2021,totally 100 dogs in western provinces of Hamedan (n=45) and Kermanshah (n=55) were examined for ectoparasites infestation. Ticks, fleas and also blood specimens were collected from individual animals and anamnestic and clinical data for each animal was recorded. Ectoparasites were identified morphologically and genomic DNA was extracted from all specimens. DNAs were tested with genus-specific primers using conventional PCR. Forty-seven out of 100 dogs (47%) were infected with haemotropic Mycoplasma i.e. Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum. DNA of the bacteria was detected in Ctenocephalides canis fleas collected from dogs in Hamedan however, Mycoplasma spp. were not detected in Rhipicephalus sanguineus sensu lato ticks in Hamedan. Present study shows for the first time, infection of dogs of Hamedan and Kermanshah provinces with haemotropic Mycoplasma species with a considerable infection rate. Application of ectoparasiticides for dogs living in these areas is advocated. Further studies are needed for better understanding of the epidemiology of this neglected zoonotic pathogen.
پژوهشگران	کیمیا بیوس (نفر اول)، علی گودرز (نفر دوم)، علیرضا سازمند (نفر سوم)