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چکیده
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Successful reproduction of the plants is considered as a critical challenge in meeting the nutritional needs of the growing world population. A major signifcant change in the plant's life cycle is the transition from vegetative to reproductive phase. The fowering and bolting processes are infuenced by several genetic and physiological factors. Molecular and genetic stud-ies have identifed key factors in age-dependent gene regulatory pathways such as miR172-miR156-SPLsmodule in phase transition in plants. Unlike in model plants, the role of miR172-miR156-SPLslike regulatory networks remains unexplored in crops such as Beta vulgaris.In the present study, for the frst time, miR156 from Beta vulgariswas identifed and character-ized. miR156gene from Beta vulgariswas cloned and ectopically overexpressed under the control of strong 35Spromoter in sugar beet explants. Subsequently, transcript levels of the miR156and its known targets (SPL4and SPL9) were quantifed in the transgenic and control plants. Consistent with reported role of mir156in downregulation of SPL4and SPL9, miR156 overexpression plants exhibited delayed fowering and atypical rooting pattern indicative of abnormal root development and poor root foraging performance. Altogether, our results suggest that Beta vulgaris miR156modulate phase transition by acting as negative regulator of SPL4and SPL9.
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