مشخصات پژوهش

عنوان	Antibiotic resistance and virulence factor gene profile of A. hydrophila isolated from carp (Cyprinidae) suspected with hemorrhagic septicemia in Gilan, Iran
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Aeromonas hydrophila, antibiotic resistance, carp, Cyprinidae, hemorrhagic septicemia, multi-drug resistance (MDR), virulence factor genes (VFG).
چکیده	The present study was conducted to determine the antibacterial resistance profile of Aeromonas hydrophila (n = 42) isolated from the 100 hemorrhagic septicemia-suspected carp in Gilan, Iran. The prevalence of class 1 and 2 integrons, antibiotic resistance genes (ARG) and virulence factor genes (VFG) among these isolates was investigated using PCR. Also, the possible association between the presence of VFGs and the antibiotic resistance profile of isolates was assessed. The majority of A. hydrophila isolates (83 33%) exhibited multidrug resistance (MDR) profile, and all isolates were resistant to clindamycin, while all isolates were susceptible to amikacin. intI1 and intI2 gene was found in 26 2 and 4 8% isolates, respectively. This is the first report of the presence of the intI2 gene in A. hydrophila isolates in Iran. The blaTEM (40 5%) and tetA (33 3%) genes were found as the predominant ARGs. The most frequently detected VFGs were lip and ahh1(90 5%), while the examined isolates carrying at least three VFGs and the most prevalent VFGs profile was ast+, act+, alt+, ahhl+, aerA+, ahyB+ and lip+. The results of this study indicate a positive association between the presence of VFGs and antibiotic resistance, and most MDR A. hydrophila isolates showed high frequencies of VFGs.
پژوهشگران	علی گودرز (نفر اول)، مرتضی یاوری (نفر دوم)، مهدی نوری کلورزی (نفر سوم)، فاطمه برزویی (نفر چهارم)، آرش منوچهری تبار (نفر پنجم)، جواد طلوعی گیلانی (نفر ششم به بعد)