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چکیده
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Aeromonads are one of the most important bacterial agents that can cause extensive economic loss and mortality in the in fish industry. Aeromonas hydrophila is the main causative agent of hemorrhagic septicemia in warm-water fishes, especially Cyprinidae. This bacterium plays an important role in public health and infection with A. hydrophila in humans through the consumption of contaminated fish meat can cause gastroenteritis, traveler's diarrhea, wound infection, pneumonia, and meningitis. Considering the importance of Cyprinidae culturing in Iran, and the significant role of A. hydrophila in infectious disease of aquaculture fishes, this study aimed to estimate the prevalence of Aeromonas hydrophila among Cyprinidae suspected with hemorrhagic septicemia in Gilan, Iran. The A. hydrophila strains were isolated and identified from the surface wounds (n=15) and kidney (n=85) of 100 Cyprinidae fishes with clinical signs of hemorrhagic septicemia, by conventional microbiological methods. Isolates suspected to A. hydrophila were confirmed by PCR using primers A16S-F and A16S- R targeting the 16S rDNA gene fragment. Among 100 specimens from Cyprinidae fishes suspected with hemorrhagic septicemia, 51 samples were positive for A. hydrophila by conventional microbiology methods. Finally, 42 isolates were confirmed as A. hydrophila by PCR technique. Due to the high prevalence of A. hydrophila in Cyprinidae suspected with hemorrhagic septicemia in Gilan province, preventive and diagnosis strategies are necessary to control the occurrence of hemorrhagic septicemia by identification A. hydrophila in fishes. Besides, in order to determine the definitive identity of A. hydrophila, in addition to culture, the simultaneous use of other methods such as PCR can be very helpful.
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