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چکیده
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Rosmarinic acid (RA) is one of the flavonoid species which is abundantly found in fruits and vegetables. Importance of RA has attracted attention of scientists, and anti-inflammatory, antioxidant, antitumor, anti-viral, anticancer and antibacterial effects of it have been reported by many of scientists [1]. Statistical analysis indicated that in Melissa officinalis L. extract, rosmarinic acid is at the highest level [2]. On the other hand, electrochemical methods are widely applied to the study of the reactions of catechol species. RA has two electroactive catechol moieties, which are independent of each other and can participate in the electrochemical oxidation-reduction process [3]. In this work at first the biosynthesis of silver nanoparticles has been performed with the leaf extract of melissa officinalis L. Synthesized nanoparticles were confirmed by UV-Visible and FT-IR spectrophotometer, XRD and SEM image. Silver nanoparticles, with large surface area, good biocompatibility, high conductivity and electrocatalysis characteristics, have been suitable for facilitate the transfer of electrons at the carbon paste electrode (CPE) [4]. In the second step, we constructed a novel, stable and sensitive electrochemical electrode based on silver nanoparticles, paraphine and graphite, as the working electrode for the electrooxidation of rosmarinic acid. Electrochemical and adsorption behaviour of rosmarinic acid at the surface of this modified electrode investigated using cyclic voltammetry and differential pulse voltammetry techniques at the biological pH.
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