مشخصات پژوهش

عنوان	Molecular Diagnosis and Pathological Study of Toxoplasma gondii in Aborted Caprine and Ovine Fetuses in Borderline of Iran–Iraq
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Toxoplasmosis, Zoonosis, Parasitic infection, Small ruminants, PCR, Histopathology
چکیده	Background Infection with the protozoan parasite Toxoplasma gondii is widely prevalent in animals and humans. In goats and sheep this infection has a high economic impact as it causes abortions and lamb losses. Although there are several stud- ies reporting seroprevalence in small ruminants in Iran, molecular-based information is scarce. Purpose This study aimed to screen caprine and ovine aborted fetuses for T. gondii infection by PCR and histopathology. Methods Brains of 121 aborted fetuses (10 caprine and 111 ovine) were collected from different parts of the Kordestan province, bordering with Iraq. Gestational age and the general status of each fetus such as freshness, autolysis, mummifica- tion and presence of macroscopic lesions in the fetus and foetal membranes was recorded. Individual brain tissues of fetuses were subjected to nested-PCR targeting the B1 gene, and histopathological sections prepared from brains were examined microscopically. Results PCR results revealed T. gondii-associated abortion in one caprine and nine ovine fetuses (8.3%). Microscopically, pathological lesions included non-purulent meningitis associated with gliosis, focal necrosis, and occasionally tissue cyst. Conclusion This paper reports for the first time T. gondii-associated abortion in goats in Iran. As organs from aborted fetuses do not necessarily show lesions, molecular confirmation is the unique diagnostic method and should be used in situations of an abortion epidemic.
پژوهشگران	ارژنگ پرتواندازان پور (نفر اول)، زینب صادقی دهکردی (نفر دوم)، لقمان اکرادی (نفر سوم)، منیره خردادمهر (نفر چهارم)، مریم رسولی (نفر پنجم)، علیرضا سازمند (نفر ششم به بعد)