مشخصات پژوهش

عنوان	Optimization Conditions for Maximum Oil Bioremediation and Biopolymer Production by Pseudomonads
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Petroleum contaminated, Bacterial growth, Crude oil , Polyhydroxyalkanoates
چکیده	The main objective of the present study was to isolate polyhydroxyalkanoate-producing pseudomonads from the petroleum hydrocarbon-contaminated area, investigate bioremediation potential of these bacteria and then optimize the culture conditions for maximum bacterial growth and polyhydroxyalkanoate production from crude oil. Forty-five oil-degrading pseudomonads were isolated and 15 polyhydroxyalkanoate-producing pseudomonads have been detected. Temperature = 30 C, incubation time = 7 days, polyhydroxyalkanoates production media and 2% (V/V) crude oil were selected as the optimum conditions for maximum bacterial growth and polyhydroxyalkanoates accumulation. Finally, the produced biopolymers were characterized by gas chromatography/mass spectrometry. Under the optimal conditions, the highest bacterial growth (optical density 600 nm) and polyhydroxyalkanoates content (% W/W) were 0.35 ± 0.05 and 22.25 ± 0.13, respectively. The present study shows that unfavorable conditions such as petroleum-contaminated area can be potential resources for polyhydroxyalkanoates producers. These isolates could be used in future bioremediation of hydrocarbons while producing industrially relevant polymers as a by-product at the optimal condition
پژوهشگران	علی گودرز (نفر اول)، محمد طباطبایی (نفر دوم)، محمد هادی اسکندری (نفر سوم)، داریوش مولا (نفر چهارم)