مشخصات پژوهش

صفحه نخست /FIRST REPORT OF BACTERIAL ...
عنوان FIRST REPORT OF BACTERIAL LEAF SPOT CAUSED BY PSEUDOMONAS SYRINGAE pv. PORRI ON ONION IN IRAN
نوع پژوهش مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها P. syringae pv. porri in Iran
چکیده In October 2016, bacterial leaf spots on onion (Allium cepaL.) were observed in fields with rainy irrigation of Eghlid, Fars province in Iran with the rate of occurrence varying from 90 to 100% of the plants. Symptoms on leaves comprised leaf blight and water soaked, irregular, white and brown spots on the leaf surface. Bacterial isolates were obtained on nutrient agar (NA) from leaf spot and blight lesions that were surface disinfected in 70% ethanol for 45 s. The isolates were fluorescent on King’s B agar, gram-negative and strictly aerobic. All isolates belonged to P. sy-ringae(LOPAT) group Ia (+, −, −, −, +) (Lelliott et al.,1996). Isolates were deposited in the Culture Collection of the Bu-Ali Sina University of Iran. DNA was extracted from the representative isolate (YMAO17) by the alkali lysis method and the 16S rRNA, gyrBand rpoDregions were partially sequenced using fD1/rP2 (Weisburg et al., 1991), gyrB-Fpsc/ gyrB-Rpsc, and rpoD-Fpc/rpoD-Rpsc (Sarkar and Guttman, 2004) primers, respectively. PCR product were sequenced and their sequences were aligned and compared with those deposited in GenBank. Sequences of the 16S rRNA (Acces-sion No. KY684039), gyrB(KY684037) and rpoD(KY684038) of a representative isolate showed 100% identity with those of Pseudomonas syringaepv. porri. To confirm Koch’s postu-lates, leaf spot symptoms were reproduced on onion leaves inoculated with bacterial suspensions (1 × 10 6 CFU/ml) and the same bacterial isolate was reisolated from artificially in-fected leaves as confirmed by LOPAT. To our knowledge, this is the first report of bacterial leaf spot of onion caused by P. syringae pv. porri in Iran.
پژوهشگران یوسف مرادی امیراباد (نفر اول)، غلام خداکرمیان (نفر دوم)، حسن رمضانی زلندی (نفر سوم)