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چکیده
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Anaplasma is obligate intracellular bacteria that lives in blood cells of mammals, particularly ruminants and is important in terms of veterinary and medical. Five species including: A. ovis, A. bovis, A. centrale, A. marginale and A. phagocyophilum can be seen in ruminants. From July 2015 to October 2015, 370 blood samples of sheep and goat were collected from different regions in Hamedan province, Iran. The DNA extracted from blood and subsequently, 16S rRNA and MSP4 genes were analyzed by Nested-PCR, Semi nested- PCR and RFLP methods. Based on blood smears microscopic observations, 97 (26.2%) of samples were infected with Anaplasma species. Also the blood samples were analyzed using PCR assessment, which Results showed that 102 (27.56%) of blood samples were positive for Anaplasma spp. and overall 1.08%, 27.02%, 0.54% of positive samples infected with A. phagocytophilum, A.ovis and A.marginale respectively. The statistical analysis (the Chi-square test) did not show any significant relation between infection and variables such as spraying, tick infestion, age
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