مشخصات پژوهش

عنوان	Growth Inhibitory Effect of Anthemis haussknechtii Root Extract, as a Source of Parthenolide, on Breast Cancer Cell Line
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Cell Viability, FT-IR; HPLC, MTT test, Parthenolide
چکیده	Parthenolide is major Sesquiterpene lactones present in Anthemis haussknechtii Boiss. & Reut. (feverfew). This compound has many effects on different disease such as migrain and cancer. Parthenolide was reported from Tanacetum parthenium (L.) Sch.Bip. but other plants of Asteraceae family could contain parthenolide. In this study parthenolide was extracted and identified with two methods, Fourier Transform Infra-Red Spectroscopy (FTIR) and High Performance Liquid Chromatography (HPLC). Breast cancer cell line, MDA-MB-231, was exposed to different concentrations of parthenolide for 24 hours. Half maximal inhibitory concentration (IC50) was evaluated using Methylthiazol Tetrazolium (MTT) test. Based on results, 1000 μg/ml concentration is the minimum lethal dose that kills approximately 50% of cells after 24 hours. The results revealed that the A. haussknechtii parthenolide dramatically decreased survival of cancer cell line by inducing apoptosis. This is the first report of cytotoxicity effect of A. haussknechtii extract on breast cancer cell line.
پژوهشگران	نسا جعفری (نفر اول)، سنبل ناظری (نفر دوم)، زهره ربیعی (نفر سوم)، ستار طهماسبی انفرادی (نفر چهارم)، رضا بهروزی (نفر پنجم)