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چکیده
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Email address: oryan@shirazu.ac.ir 1st Congress of Infection and Immunity September 28-29, 2016 28 Molecular Biology and Microscopical identification of Anaplasma spp in Cattle, in Talesh- Golestan province Sadeghi Dehkordi Z¹; Salehi Guilandeh S²; Sadeghinasab A³; Azami S 4 1 Department of Pathobiology, Faculty of Para Veterinary Science, Bu Ali Sina University, Hamedan, Iran 2 Faculty of Para-Veterinary Science, Bu-Ali Sina University, Hamedan, Iran 3 Department of Clinical sciences, Faculty of Para Veterinary Science, Bu Ali Sina University, Hamedan, Iran 4 Department of Pathobiology, Faculty of Para Veterinary Science, Bu Ali Sina University, Hamedan, Iran Anaplasmosis is a vector-borne, infectious blood disease in cattle caused by the rickettsial parasites Anaplasma marginale and Anaplasma centrale. It occurs primarily in warm tropical and subtropical areas. The disease is not contagious but is transmitted most commonly by ticks. The intracellular parasite destroys red blood cells. It causes anemia, fever, weight loss, breathlessness, uncoordinated movements, abortion and death. Diagnosis is based on clinical signs and the examination of blood under microscope for evidence of the parasite. From March 2015 to August 2015, 150 blood samples and corresponding blood smears of cattle without any signs of disease were prepared from a region in Talesh ( Golestan province), randomly. The blood smears were first stained with Giemsa and analyzed for the presence of Anaplasma spp. The extracted DNA from blood cells were analyzed by A. marginale and A. centrale by specific nested PCR using primers derived from the msp4 gene and specific nested PCR using primers derived from 16SrRNA gene, respectively. 100 blood smears were positive for Anaplasma spp. (66.6%) and 50 samples (33.3%) were negative by Giemsa staining, but 18 out of 150 blood samples (12%) were positive for A. central by specific nested PCR using specific primers derived from 16S rRNA gene and 14 blood samples
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