مشخصات پژوهش

عنوان	Incidence, distribution, economic importance of alfalfa witches’ broom disease in Sistan-Baluchestan (Iran) and characterization of associated phytoplasmas
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	PCR, RFLP, phytoplasma group, Medicago sativa L
چکیده	Alfalfa witches’ broom (AWB) is a devastating disease distributed mainly in tropical provinces of Iran and widespread all over the Sistan-Baluchestan province. The highest incidence was found in Rask (Sarbaz) (70%), Sefidaba (Zabol) (46.26%), Sarboog (Nikshahr) (45.4%), Mirjaveh (Zahedan) (40%), Shams Abad (Saravan) (32.2%), Namardian (Iranshahr) (27%), Kahir (Chabahar) (25.7%) and Najaf Abad (Khash) (17.8%). Dodder inoculation of periwinkle plants with AWB agents from different areas induced undistinguishable symptoms. Detection and identification of AWB associated phytoplasmas was based on characteristic disease symptoms and direct and nested polymerase chain reaction using P1/P7, R16mF2/ R16mR2 and R16F2n/ R16R2 primer pairs. RFLP and phylogenetic analyses of 16S rDNA showed that AWB associated phytoplasmas from Sistan-Baluchestan belong to peanut witches’ broom (16SrII) group. Virtual digestion of R16F2n/R16R2 sequence of a Nikshahr AWB strain exhibited RFLP profiles referable to those of members of the 16SrII phytoplasma group, subgroup D
پژوهشگران	سیدعلیرضا اسمعیل زاده حسینی (نفر اول)، غلام خداکرمیان (نفر دوم)، محمد صالحی (نفر سوم)، سید رضا فانی (نفر چهارم)، سید مهدی میر چناری (نفر پنجم)، آسونتا برتاچینی (نفر ششم به بعد)