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چکیده
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Background: Urinary Tract Infections or UTIs is considered one of the most common bacterial infections that approximately 50-80% of them are caused by Uropathogenic Escherichia coli (UPEC) strains. One of the main factors causing disease of (UPEC) strains is Haemolysin gene, Thecytolytic protein toxin secreted by most hemolytic E. coli strains is known as alphahemolysin (hlyA). Haemolysin molecules insert into lipid-containing membranes and lysing them. Four genes are required for the production and export of the haemolysin that hlyA gene is the most important them. Objectives: Haemolysin production is associated with human pathogenic strains of E. coli especially those leading to more clinically severe forms of UTI. Hence, the identification of specific areas with a high prevalence of gene prevents the exploitation of different vaccines and reduces the high costs. Methods: From 80 collected samples, Fifty E. coli isolates from patients with UTI, isolated at the cell and molecular lab, IAUB, Boroujerd. E. coli presence confirmed by biochemical methods. Then DNA extraction performed by kit protocol and designed specific primers used to PCR. Forward primer was F:5'- AACAAGGATAAGCACTGTTCTGGCT-3' and Reversed primer was R: 5'- ACCATATAAGCGGTCATTCCCGTCA-3'. The apmplicon size was 1177 bp. Result: Among of 50 E. coli isolated, 18 isolates expressed HlyA gene (36%) included 11 women and 7 men. Conclusions: HlyA gene expression levels was relatively high among both sexes, and the findings are consistent with previous studies; therefore, there is supposed a key role for this gene in the incidence of urinary tract infections. our result also can be used for produced local vaccines.
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