مشخصات پژوهش

عنوان	Developing an indirect ELISA test using whole tachyzoite for sero-diagnosis of Neospora caninum in water buffalo from north-west of Iran
نوع پژوهش	مقاله ارائه شده کنفرانسی
کلیدواژه‌ها	ELISA, Neospora caninum, water buffalo, tachyzoite, Iran
چکیده	Background: Neospora caninum is an obligate intracellular parasite introduced in 1988. It can infect water buffalo (Bubalus bubalis) inducing abortion and causing reproductive failure. Developing simple methods with high accuracy for detecting the infection has always been a goal for researchers. Methods: To assess the prevalence of N. caninum antibodies in buffalo, a new ELISA-based method was established using the whole parasite. 83 serum samples were collected from buffaloes slaughtered in Urmia abattoir. Plates were coated with 2×106 whole tachyzoites and incubated at 25°C for 3 days. After washing and blocking with relevant buffers, serum samples as well as negative and positive controls were applied. HRP conjugated anti-bovine IgG and tetramethylbenzidine substrate were used to detect the bound antibodies. Optical density (OD) values were measured and the antibody level was determined by calculating the ratio of sample/positive control (s/p) ODs and those with the ratio more than 0.50, were accounted as positive Results: A total of 83 serum samples were examined for presence of anti N. caninum IgG. 16 samples (19.27%) were positive from which 3 were mail and 13 were female. 33(39.75%) showed a significant level of antibody where there s/p ratio were below 0.50. No significant level of Antibody was observed in 34(40.96%) samples Conclusion: Whole N. caninum tachyzoites can be used as antigen for detection of the parasite in an ELISA-based assay. There is a high level of infection in water buffalos, which indicates a necessity for application of control strategies in this merit livestock.
پژوهشگران	رویا عابدی زاده (نفر اول)، حسین رضوان (نفر دوم)، محمد نام آوری (نفر سوم)، علی خاکی (نفر چهارم)، سکینه اعظمی (نفر پنجم)