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Abstract
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The torsion of the testicle is one of the urological emergencies that often occurs in children. Early diagnosis and detorsion surgery are very important in preventing testicular tissue damage and resulting infertility. This study aimed to investigate the effects of the hydroalcoholic extract of Malva sylvestris on the sperm parameters in mature mice following testicular torsion. In this study, 24 male mice were randomly divided into 4 groups (n=6) including the control group, where no intervention was performed, the torsion/detorsion group, in which the left testicle along with the spermatic cord was rotated 720 degrees counterclockwise for 1 hr, the group received M. sylvestri extract at a dose of 200 mg/kg and the treatment group received the extract through gavage for 35 days after torsion/detorsion. The comparison of sperm parameters in all groups indicated that sperm count, viability, motility, maturity, and DNA integrity were significantly decreased in torsion/detorsion group compared to the other groups. In torsion/detorsion + M. sylvestri group, all above-mentioned sperm parameters were significantly increased and improved compared to the torsion/detorsion group, but had significant differences with control and M. sylvestri groups. The percentage of abnormal sperm was significantly decreased in torsion/detorsion group compared to the other groups. In torsion/detorsion + M. sylvestri group, abnormal sperm percentages were significantly decreased compared to the torsion/detorsion group, but had significant differences with control and M. sylvestri groups. In conclusion, the use of M. sylvestirs extract due to its anti-oxidant properties can be an approach to reduce complications caused by ischemia/reperfusion injuries.
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