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Abstract
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Aims: Methotrexate (MTX) has a unique position in the treatment of various disorders such as RA and has disadvantages such as nephrotoxicity. Disadvantages and long-term side effects of MTX are the main causes of discomfort for patients. Therefore, new approaches are needed to improve patient tolerance and reduce side effects of MTX. Therefore, considering the antioxidant effects of coenzyme Q10, this study was conducted with the aim of investigating the protective effects of coenzyme Q10 in the kidney tissue of mice treated with methotrexate Material & Methods: In this experimental study, 24 male NMRI mice were randomly divided into 4 groups (n=6). Control group: 0.2 ml of distilled water, MTX group, methotrexate at a dose of 20 mg/kgBW once a week by intraperitoneal injection, CoEnQ10 group: coenzyme Q10 at a dose of 200 mg/kgBW three times a week orally and MTX+CoEnQ10 group: methotrexate (20 mg/kgBW-IP) once a week and coenzyme Q10 (200 mg/kgBW-Po) three times a week. All groups were treated for five weeks. After euthanizing, epididymal tails in order to evaluate the sperm parameters and testes, in order to investigate histological and morphometrical alterations and spermatogenesis index, were collected in different groups. Results: The evaluation of the tissue changes in the kidney showed that methotrexate had caused necrosis, inflammation, destruction of renal corpuscles and hyperemia in the kidney, and the use of coenzyme Q10 could partially resolve these complications. Also, the study of kidney histomorphometry revealed that the consumption of methotrexate caused a significant decrease in the diameter of the glomerulus, Bowman's capsule, proximal and distal convoluted tubules, thin and thick sections of the tube of Henle, and the urine collecting tube (P < 0.05) and consumption of coenzyme Q10 along with methotrexate was able to significantly improve these parameters (P < 0.05). Conclusion: According the results obtained in this study, it can be concluded that the use of coenzyme Q10, as an antioxidant in inhibiting lipid peroxidation and DNA oxidation, can reduce the adverse effects of methotrexate in kidney tissue.
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