|
Title
|
Organogenesis from seedling derived leaf explants of primrose (Primula heterochroma Stapf.) as a scarce Iranian plant
|
|
Type
|
JournalPaper
|
|
Keywords
|
Endemic plant; In vitro culture; Plant growth regulators; Shoot regeneration; Thidiazuron.
|
|
Abstract
|
A simple regeneration method is reported for Primula heterochroma, a rare plant, endemic to the North of Iran. The seedling derived leaf explants were cultured on several Murashige and Skoog (MS) basal media enriched with different concentrations of 6- benzylaminopurine (BA) or Thidiazuron (TDZ) in combination with different concentrations of 1-naphthaleneacetic acid (NAA). The results showed that the highest frequency of shoot regeneration was 93% and 94% on the media containing 2 mg.l–1 BA + 2 mg.l–1 NAA and 2 mg.l–1 TDZ + 1 mg.l–1 NAA, respectively, and the largest number of shoots per leaf also were induced in abovementioned concentrations. Adventitious shoots were regenerated directly from leaf explants in all responsive explants. Regenerated shoots easily rooted without the necessity for transferring to an additional rooting medium. A method described in this paper may offer the potential of being applied to other endangered Primula spp
|
|
Researchers
|
Hedayat Zakizadeh (Third Researcher), Alireza Noroozisharaf (Second Researcher), Yousef Hamidohli (First Researcher)
|