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Title
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In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat
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Type
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JournalPaper
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Keywords
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wound healing, Achillea millefolium, essential oil, antibacterial
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Abstract
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Objectives In this study we aimed to evaluate the in vitro antibacterial activity and wound healing properties of Achillea millefolium essential oil (AMEO) in full-thickness wound model in rat. The antibacterial activity of AMEO was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa using the broth dilution method. Methods The 2 cm × 2 cm full-thickness excisional wounds were created on the back of animals. Topical therapy was applied twice a day using 1%, 2%, and 3% w/w AMEO ointments, and the measurement of the wounds area was carried out every 3 days, after that the wound closure percentage was calculated in these days. Hydroxyproline content and histopathological evaluation of wound tissue samples were carried out on day 7 and 14 post wounding. Eucerin was used for the treatment of vehicle control group and negative control group received no treatment. Results Our results revealed the bacteriostatic activity of AMEO against S. aureus and P. aeruginosa. Wound healing activity evaluation of AMEO showed the significant increase (p < 0.05) in the wound closure percentages in rats treated with AMEO 1% and 2% comparing to those of non-treatment group. In addition, hydroxyproline contents of tissue significantly (p < 0.01) increased in AMEO 1% and 2% comparing to non-treatment group. Histopathological evaluations of wound tissue samples on day 7 and 14 demonstrated higher accumulation of collagen fibers, reduction of edema and inflammation and also formation of tissue appendages in 1% and 2% AMEO treated groups in comparison with non-treatment group. Conclusion The results of this study indicated that AMEO has the potential to be used as a safe and effective wound healing agent.
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Researchers
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Dara Dastan (Not In First Six Researchers), Alireza Nourian (Fifth Researcher), Shabnam Pourmoslemi (Fourth Researcher), Pari Tamri (Third Researcher), Akram Ranjbar (Second Researcher), Mohammad Reza Ghasemi (First Researcher)
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