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Abstract
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Objectives: The aim of this experiment was to estimate relative bioavailability of a type of organic DCP, which biological usability in poultry feeding has not been determined so far. Materials & Methods: Inorder to estimate bioavailability of DCP in organic source (19.5% phosphorus) compared to standard source (phosphoric acid 85%, containing 26.87% phosphorus), 280 one-day-old male broiler chickens of Ross 308 were used in a completely randomized design with 7 treatments, 4 replications and 10 observations per replicate for 35 days. Phosphorus from two sources of phosphorus supply at three levels (0.15%, 30% and 45% of the diet) was added to basal diet containing 15% available phosphorus. To estimate relative bioavailability of available phosphorus in tested DCP source, the slope ratio method was used in SAS software. Based on slope ratio method, linear dependence of dependent variables (body weight, feed conversion ratio, serum alkaline phosphatase, breaking force and length and percentage of bone ash) were calculated from independent variable (phosphorus added levels). Results & Conclusion: Details related to examination of three prerequisite hypotheses for estimation of relative bioavailability in tested substance included estimation of line slope, standard error of line slope, relative bioavailability, width from origin and coefficient of determination. Probability level was also calculated for linear subordination, non-linear subordination (quadratic), difference in width from origin of two lines, difference in width from origin of zero and estimated level, difference in slope of two lines and finally effect of available phosphorus level of source added to basic ration. All mentioned items were calculated for body weight, feed conversion ratio, serum alkaline phosphatase logarithm, tibia breaking force logarithm, tibia length logarithm, tibia diameter logarithm, tibia ash content and tibia ash phosphorus content. Comparison of DCP bioavailability with standar
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