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Abstract
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Monitoring the levels of anticancer drugs in real samples would allow dose optimization. Mesna is the sodium salt of 2-mercaptoethane sulfonate, a thiol-containing drug. MESNA was proved to be effective in preventing hemorrhagic cystitis induced by high doses of several chemotherapeutic regimens such as cyclophosphamide and ifosfamide. Because of the increased level of interest in using Mesna for treating various disorders, a new and sensitive method was needed to understand the pharmacokinetics of this drug. Therefore, the determination of Mesna in real samples is very important and essential [1]. Electrochemical impedance spectroscopy (EIS) is a powerful method to investigate the surface of electrodes in detail. many EIS-based electrochemical sensors are based on measuring the charge transfer resistance (Rct) of redox probes at the surface of electrodes to monitor the binding of target molecules have been reported [2]. Mesna determination by conventional electrochemical methods was not reported. In this research, we developed a novel impedimetric sensor based on glassy carbon electrode (GCE) modified with oxidized multiwalled carbon nanotubes (MWCNTs)/gold nanoparticle(AuNPs) (denoted as Au NPs/MWCNTs/GCE) for impedimetric determination of Mesna anticancer drug. The modified electrode was characterized by field emission scanning electron microscopy (FESEM), energy dispersive Xray (EDX), and EIS. The EDX spectrum confirmed that the presence of carbon, gold and oxygen elements in the prepared composites.The detection mechanism of the proposed impedimetric sensor was based on the increase in the [Fe(CN)6] 3-/4- charge transfer resistance (RCT) as an electrochemical probe in the presence of the target analyte. Under the optimum condition, the calibration curve at the Au NPs/MWCNTs/GCE covered two linear dynamic ranges, 0.06 1.0 and 1.0 130000.0 nM, with a detection limit of 0.02 nM. Eventually, in order to test the analytical investigation, the practical application
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