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Abstract
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Over the past few decades, multifunctional hybrid bio-nanomaterials have emerged as nanozyme antioxidants, which are a new interdisciplinary frontier between nanotechnology and life sciences. In this study, we have successfully synthesized a novel Pt-cyclopropa[60]fullerene complex (C60/Pt–I–Cl) incorporating a-keto stabilized phosphorus ylide in a three-component reaction of the unsymmetrical phosphorus ylides [Ph2P(CH2)PPh2=C(H)C(O)C6H4–m–Cl], C60 and Pt2(dba)3 (dba = dibenzylideneacetone). In the next step, by immobilizing this complex on graphene oxide (GO) nanosheets, the novel GO-Pt nanozyme (C60/Pt–I@GO) was synthesized. Finally, cytotoxicity of the developed C60/Pt– I@GO was examined by MTT assay against NIH3T3 cells. According to the results, this nanocomposite showed suitable cytocompatibility even at a relatively high concentration of the sample (25 lg mL-1) in both 24 and 48 h. In detail, the viability of the cells for the highest concentration was obtained as 93.7 and 90.2 after 24 and 48 h, respectively. The a,a-diphenyl-1-picrylhydrazyl (DPPH) assay was applied to evaluate the antioxidant activity of C60/Pt–I@GO nanocomposite. The received result not only did signify the antioxidant activity of this nanocomposite but also revealed a clear dose-dependent antioxidant activity for the produced nanocomposite. Also, the result of the time-dependent evaluation of C60/Pt–I@GO nanocomposite antioxidant activity could confirm that they could reduce DPPH overtime to reach almost a plateau after 6 h of treatment, which could reveal the persistence of C60/Pt–I@GO nanocomposite to function effectively over time. Overall, C60/Pt–I@GO nanocomposite was successfully produced and found effectively functional in reducing the DPPH radical, so they could be considered as a promising candidate to be studied as a novel nanozyme antioxidant.
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