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Title
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DNA-functionalized gold nanoparticles for molecular diagnosis of human viral pathogens
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Type
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Presentation
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Keywords
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gold nanoparticles molecular diagnosis viral pathogens conventional polymerase chain reaction (PCR)
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Abstract
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DNA-functionalized gold nanoparticles which are called spherical nucleic acids (SNAs) are an emerging class of three-dimensional nanostructures created by immobilizing a dense shell of DNA or RNA around a nanoparticle core. SNAs exhibit unique properties different from those of both individual nanoparticle core and nucleic acid shells. Colorimetric and fluorometric biosensor development based on AuNP-core SNAs is a theme that runs through merging the distance-dependent plasmonic properties of AuNPs and the programmable nature of DNA-DNA interactions. This study centers on utilizing DNA-programmable assembly of gold nanoparticles as optical transduction in nanobiosensing processes. We developed colorimetric and fluorometric biosensing platforms based on DNA-programmable assembly of gold nanoparticles for molecular diagnosis of human viral pathogens. SNA-based assays have the potential to be especially useful for point-of-care testing by overcoming the challenges provided by specialized apparatus. We coupled SNA probes with conventional polymerase chain reaction (PCR) through 5′-exonuclease activity of the DNA polymerase to modify PCR assay for the naked-eye colorimetric detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. Due to public health interest in diagnosing infectious diseases using simple, accessible, and affordable laboratory equipment, the biosensing method based on the programmable assembly of SNA may be envisioned to have widespread applications in the diagnosis of COVID-19 and other infectious diseases.
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Researchers
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abbas karami (First Researcher), Masoumeh Hasani Mousavi (Second Researcher)
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