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Abstract
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Induction of gynogenesis through ovule culture is a valuable tool to produce haploid and doubled haploid plants in sugar beet (Beta vulgarisL.). However, there is still large room for refning the method. In this study we investigated the gynogenic response of cultured ovules of three sugar beet genotypes, the efect of the application to inforescences of diferent pretreat-ments with mannitol at 4ºC and with 5-azacytidine and 2,4-D, and the efect of the use of diferent basal culture media and sucrose concentrations. The response was evaluated in terms of percentages of induction of gynogenesis, embryogenesis and callogenesis, as well as of regenerated plants. We showed that a pretreatment with 0.5 M mannitol at 4 °C for 4 days, and with 50 µM 5-AzaC for 1 h, notably improved the percentage of embryogenesis and plant regeneration. Besides, the use of MS basal medium and 60 g/L sucrose was also found benefcial. This study provides new ways to improve the efciency of haploid induction and plant regeneration through ovule culture in sugar beet, and is potentially applicable to ovule culture in other crops.
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