Research Info

Title	Phytochemical, antioxidant, enzyme activity and antifungal properties of Satureja khuzistanica in vitro and in vivo explants stimulated by some chemical elicitors
Type	JournalPaper
Keywords	Methyl jasmonate; multi-walled carbon nanotubes; nodal segments culture; rosmarinic acid; salicylic acid
Abstract	Context:Satureja khuzistanica Jamzad. (Lamiaceae), is known for its antifungal and antioxidant com-pounds, especially rosmarinic acid (RA). Objective:The study examines the effect of elicitors on RA production and phytochemical properties of S. khuzistanica. Materials and methods:In vitro plants were treated with methyl jasmonate (MeJA) and multi-walled car-bon nanotubes (MWCNTs).In vivo plants were treated with MWCNTs and salicylic acid (SA). RA was meas-ured by HPLC. Catalase (CAT), guaiacol peroxidase (POD) and ascorbate peroxidase (APX) were quantified. DPPH andb-carotene were assayed in in vivo extracts. The antifungal effects of extracts were evaluated againstFusarium solaniK(FsK). Results:The highest RA contents ofin vitro plants were 50mg/L MeJA (140.99mg/g DW) and 250mg/L MWCNTs (140.49mg/g DW). The highestin vivo were 24h MWCNTs (7.13mg/g DW) and 72h SA (9.12mg/g DW). The maximum POD and APX activities were at 100mg/L MeJA (5 and 4mg protein, respectively). CAT had the highest activities at 50mg/L MeJA (2mg protein). DPPH and b-carotene showed 50% and 80% inhibition, respectively. The FsK aggregation was the lowest forin vitro extract in number of conidia [1.82 10 10 ], fresh weight (6.51g) and dry weight (0.21g) that proved RA inhibitory effects. The callus reduces FsK growth diameter to 2.75 on the 5th day. Discussion and conclusions:Application of MeJA, SA, and MWCNTSs could increase RA inS. khuzistanica and highlighted potential characteristics in pharmaceutical and antifungal effects.
Researchers	Kalliope Papadopoulou (Fifth Researcher), Dara Dastan (Fourth Researcher), Asghar Mirzaie-asl (Third Researcher), mohammad reza Abdollahi (Second Researcher), (First Researcher)