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Abstract
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Background: Methandrostenolone as Anabolic-androgenic steroid ranks among the drugs most widely abused with the goal of improving athletic ability, appearance, or muscle mass. This drug was developed as synthetic analog of testosterone and are administrated for treating refractory anemia, hereditary angioedema, breast cancer and starvation states. The doses of Methandrostenolone used by athletes is typically in excess of therapeutic doses. The aim of this study was to determine the effects of high doses of Methandrostenolone on sperm characteristics. Materials and Methods: This study was carried on 18 adult male mouse. Animals were divided to 3 equal groups as follow: control, M10 and M20 groups that respectively received 0.2 ml distilled water, 10 mg/kgBW and 20 mg/kgBW Methandrostenolone daily through gavage for 42 days. After treatment period, animals were euthanized by cerebrospinal dislocation and dissected. Cauda epididymis was collected and put in PBS buffer, then cut into pieces. Finally, sperm motility, vitality and count were evaluated. one drop of sperm suspension was spilled on the slide and sperm motility was evaluated with microscope equipped to hotplate and the result was expressed as percentage of motile sperms. Also, eosin-nigrosin staining was used for assessment of sperm vitality and sperm count was performed with hemocytometer. Results: Evaluation of sperm quality in all groups indicated that M10 and M20 groups caused significant decline in sperm quality in comparison with control group. Also M20 group showed significant decrease in sperm motility, vitality and count compared with M10 group. Conclusion: Regarding to the considerable diminution of sperm quality in animals induced with high doses of ethandrostenolone, it may affect the fecundity of the users. So, we suggest specially to the athletes to eschew for applying high doses of Anabolic Steroid Methandrostenolone.
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