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Abstract
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The walnut leaf gall mite Aceria tristriatus Nalepa is one of the major pests of walnut in western Iran. Host plant resistance has not been explored as a method for the integrated mite management of walnut leaf gall mite (Javdi-Khedri, et al., 2014). In this order the resistance of 10 native and non-native walnut cultivar genotypes including Lara, Hartly, Chandler, Jamal, Pedro, Franqutte, Tahghighat, Z60, Z53 and B21 as well as seedling were preliminary evaluated under same conditions using Antixenosis, Antibiosis and injure tests. In this study, wax amount, total phenol, flavonoid, juglone, total carbohydrate, total protein, PPO and POD enzymes activities, DPPH and FE with above mentioned cultivars and genotypes resistance to walnut leaf gall mite were evaluated. In addition, photosynthetic pigments were assessed in order to evaluate plant injury. In free choice assay, significant differences were observed in mite performance on Seedling, Hartly, Lara and Z60 leaves while Chandler and Jamal leaves contained a significant lower number of the mites. Seedling and Hartly allowed the highest mite density within the galled leaves whereas Jamal and Chandler showed the lowest mite density. In addition, the highest percentage of infestation occurred again on Seedling, Hartly, Lara and Z53 while the lowest one was observed on Chandler and Jamal cultivars. Regarding biochemical content, nearly all of the above mentioned biomarkers showed negative correlation with mite infestation. Generally, resistance cultivars such as Chandler, Jamal and Pedro significantly produced defensive compounds more than control after mite infestation, while this procedure in susceptible cultivars such as Seedling, Hartly and Lara were recorded in low amount. Amounts of photosynthetic pigments containing Chlorophyll a and b as well as carotenoid significantly were reduced in sensitive cultivars after manipulation. In conclusion, the current experimental model screened preliminary a three cultivars
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