|
Abstract
|
Background: Cutaneous Leishmaniasis (CL) is assumed as neglected tropical disease, which spontaneously heals less than one year� However, the lesions of CL rarely persist more than one year� The persistency of the lesions could be attributed to different factors, including pro-inflammatory cytokines� In this context, the role of IL-4 and IFN-gamma in duration of lesion healing has been evaluated in some of the previous studies; however the role of IL-17a in duration of healing lesion is not completely understood� The current study aims to assess the role of IL-17a in non-healing form of CL (NHCL)� Methods: This was a cross-sectional study implemented from 2015-1015 in immunodeficiency research center, Isfahan University of Medical Sciences (IUMS)� This study included 10 cases afflicted by NHCL and 33 cases suffering from healing form of CL� The peripheral blood mononuclear cells (PBMCs) of the patients were isolated by Ficoll and were harvested in three different medium� The stimulators were as follow: purified protein derivative (PPD), Phytohemaglotinin (PHA), and Soluble Leishmania Antigen (SLA)� The supernatant was isolated and the levels of IL-17a were determined using ELISA� Results: The levels of IL-17a produced in PPD stimulated wells was significantly lower, compared with PHA and SLA stimulated wells (P<0�05)� Furthermore, the level of IL-17a produced in PHA stimulated wells was significantly higher, compared with PHA stimulated wells (P<0�05)� The level of IL-17a produced in NHCL cases was significantly lower than healing cases (P<0�05)� Conclusion: Our findings support the notion that IL-17a plays a crucial role in the duration of lesion healing in those afflicted by CL� Keywords: Cutaneous Leishmania
|