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Abstract
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Existing research recognizes the critical role played by MicroRNAs (miRNAs) in regulation of gene expression in diverse cellular pathways. More recent evidence reveals that miR-20a has a key role in transition through G1 in diploid human cells. The molecular basis of miR-20a is still poorly understood . The aim of this essay is to explore the relationship between miR-20a and putative mechanism with transcription factors and its expression by bioinformatics analysis . Detailed analyze showed that has-mir-20a is an intergenic miRNA . The analyses were resume using Ensemble database and reveled that promoter region of has-mir-20a was predicted to be50 bp. CpG island was investigated using EMBOSS spgplot with default parameters (Criteria used: Island sized > 100 bp, GC% > 50.0%, Obs/Exp > 0.6)and No CpG island found in the has-mir-20a promoter region . Seventeen transcription factor binding sites was find in the promoter region including (p53, c-Myb, Pax-5, NF-1, AP-1 etc.) by different type of analytical software such promo and neural network algoritms. In our study The predictions of transcription factor binding sites ,cpg island analysis, and target gene of has-mir-20a provided significant data for further study of putative mechanisms Involved in CRC . This study has found that tumor suppressor gene such p53 contract with the putative promoter of has-mir-20a .also methylation probably has not effect on its expression. There is abundant room for further investigation in determining has-mir-20a as efficient biomarker in CRC.
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