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Title
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Virulence gene expression analysis of Staphylococcus aureus in treatment with Thymus daenensis L essential oil
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Type
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Presentation
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Keywords
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Staphylococcus aureus, Thymus daenensis essential oil, Virulence factor
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Abstract
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Staphylococcus aureus is an important human and animal pathogen that is responsible for a range of diseases that vary widely in clinical presentation and severity. Pathogenicity of this organism is attributed to various virulence factors. Even though, Thymus daenensis essential oil (EO) is well known for its antimicrobial activity against several important pathogens including S. aureus, the precise mechanism of antimicrobial action is not completely known. According to this fact the aim of present study was to evaluate the virulence gene expression of S. aureus when treated with sub-minimum inhibitory concentrations (sub-MICs) of T. daenensis EO. The inhibition potential of Quorum sensing (QS), NorA efflux pump and exopolysaccharide production of T. daenensis EO was evaluated by quantitative Real-Time PCR (qRT-PCR) analysis. Target genes in present study were RNAIII (for QS), norA (for efflux pump) and icaA (for exopolysaccharide). 16S rRNA gene was used as reference for normalization of results. The relative gene expression was analyzed using the 2-ΔΔCT method. The MIC value of EO for S. aureus ATCC 25923 was 0.5 μl.ml-1. The results of qRT-PCR analysis showed that at MIC/2 (0.25 μl.ml-1) concentration, this EO was able to decrease more than a 2.5 fold in the gene expressions of the all three investigated genes. The production of exopolysaccharide, QS functional molecule and NorA efflux pump protein in S. aureus was decreased by T. daenensis EO. These data suggest that T. daenensis EO may be useful for the treatment of S. aureus infections.
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Researchers
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Bahar Nayeri Fasaei (Fifth Researcher), Taghi Zahraei Salehi (Fourth Researcher), Pezhman Mahmoodi (Third Researcher), Abdolmajid Mohammadzadeh (Second Researcher), aram Sharifi (First Researcher)
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