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Title
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Enzymatic biosensor based on entrapment of d-amino acid oxidase ongold nanofilm/MWCNTs nanocomposite modified glassy carbonelectrode by sol-gel network: Analytical applications for d-alanine inhuman serum
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Type
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JournalPaper
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Keywords
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Biosensor, d-Amino acid oxidase, MWCNTAu nanostructures, Enzyme immobilization
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Abstract
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Sensing and determination of d-alanine is studied by using an enzymatic biosensor which was constructedon the basis of d-amino acid oxidase (DAAO) immobilization by sol-gel film onto glassy carbon elec-trode surface modified with nanocomposite of gold nanofilm (Au-NF) and multiwalled carbon nanotubes(MWCNTs). The Au-NF/MWCNT nanocomposite was prepared by applying the potentiostatic techniquefor electrodeposition of Au-NF on the MWCNT immobilized on glassy carbon electrode surface. Themodified electrode is investigated by scanning electron microscopy (SEM), energy dispersive X-ray spec-troscopy (EDX), linear sweep voltammetry (LSV) and cyclic voltammetry(CV) techniques. The linearsweep voltammetry was used for determination of d-alanine and the results showed an excellent linearrelationship between biosensor response and d-alanine concentration ranging from 0.25 M to 4.5 Mwith correction coefficient of 0.999 (n = 20). Detection limit for the fabricated sensor was calculated about20 nM (for S/N = 3) and sensitivity was about 56.1 AM−1cm−2. The developed biosensor exhibited rapidand accurate response to d-alanine, a good stability (4 weeks) and an average recovery of 98.9% in humanserum samples.
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Researchers
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Amir Abbas Rafati (Second Researcher), (First Researcher), (Third Researcher)
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