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Abstract
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Abstract Background: Leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries in the world. Characterization of inflammatory responses pro-duced in cutaneous leishmaniasis has not yet been completed. Methods: The specific primers were designed for ten pro-inflammatorygenes including CCL4, CCL3, TNF-α, IL-1α, IL-12P35, IL-12P40, CCL5, CCR5, IL-1β and IFN- γ and their expression were assessed and compared using RT-PCR in the lesion and peripheral blood neutrophilsin Leishmania infected BALB/c mice. Results: None of the pro-inflammatorygenes was expressed in the healthy tissue and except IFN-γ others were down-regulated by the parasite in the lesion in untreated mice. In mice treated with anti-leismanial drugs, the expression of the pro-inflammatorygenes restarted. The figure of pro-inflammatorygene expression in neutrophils was different was from the lesions in treated and untreated mice. Conclusion: Leishmania is capable to suppress the expression of pro-inflammatory genes in the lesions but not in neutrophils. The expression of TNF-α in the lesions and down-regulation of IL-1β in neutrophils could be accounted as an indication for healing of cutaneous leishmaniasis. The results open a new window on characterization of Leishmania lesions and clarifying the role of neutrophils in Leishmania infections.
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