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Title
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Thymus denensis essential oil as new Quorum Sensing Inhibitors for Staphylococ-cus aurous
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Type
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Presentation
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Keywords
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Staphylococcus aureus, Quorum Sensing, Thymus daenensis
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Abstract
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Introduction and Objectives S. aureus is a significant human pathogen that causes nosocomial and community-acquired infection. Pathogenicity of this organism is attributed to various virulence factors. In S. aureus cell-cell communication via quorum-sensing (QS) systems affects the expression of these virulence factors; thus, the targeting of quorum-sensing systems with so-called quorum-sensing inhibitor (QSI) has been suggested as an alternative means of treating S. aureus infections. Materials and Methods Quorum sensing inhibitor activity was investigated by both phenotypic and genotypic methods. For evaluation of QSI we used of sub-minimum inhibitory concentrations of Thymus daenensis essential oil against S. aureus ATCC 25923. (MICs were determined by the microtiter broth method). In phenotypic technique the concentration of δ-toxin (as QS activity) was measured by RP-HPLC. In genotypic method, for the quantification of hla gene expression (α-hemolysin levels) we used real-time qRT-PCR. The 16S rRNA reference gene was used for normalization of the results. Results In both HPLC and qRT-PCR method Thymus daenensis essential oil exhibited a dose-dependent response in the production of δ-hemolysin, indicating strong anti-QS activity S. aureus. Conclusion These results support Thymus daenensis essential oil as a novel drug candidate and an effective treatment strategy for S. aureus infections.
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Researchers
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Taghi Zahraei Salehi (Fourth Researcher), Pezhman Mahmoodi (Third Researcher), Abdolmajid Mohammadzadeh (Second Researcher), aram Sharifi (First Researcher)
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