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Abstract
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Introduction & Objectives; Toxoplasma gondii is an obligate intracellular protozoan, in the Apicoplexa phylum, causes Toxoplasmosis, which is the main cause of abortion and congenital infection in sheep and goats. Various techniques are used to detect the pathogen in infected sheep fetus, one of the most sensitive and more specific diagnostic technique is modified Nested-PCR using gene B1 organism. The purpose of this study was to determine toxoplasmic abortion in sheep and goat by using PCR and histopathological tests in years 2012-2013 in the province of Kurdistan. To identify examples of positive and negative after PCR amplification of the first and second PCR products on an agarose gel (2%), electrophoresis ( the first step to identify the band 193bp and secondly to identify band 96bp) revealed 10 positive samples, the first step 3 samples were 193b band and the second band was 96bp.96bp band of the samples were positive only in the second phase and negative examples. 8.3 % of sheep and 10% of fetal goat embryos were infected with T. gondii. All positive samples were isolated from embryos that have more than 2.5 months of age. In positive PCR sample, histopathological evaluation, for presence of cysts or non suppurative encephalitis were implemented. In two cases, symptoms such as edema, hyperemia, gliosis, non purulent meningitis was found ,andin negative PCR result, cases in histopathological studies, too, no cysts was found.According to these results Toxoplasma gondii is known as a cause of abortion in this province due to prevalence of infection in livestock such as sheep and goast, and also as a source of infection in human
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