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Abstract
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Background and Aim: Pseudomonas aeruginosa produces four characteristic diffusible pigments including : pyocyanin, pyorubrin, pyoverdin and pyomelanin. Pyocyanin is the main pigments which importance during clinical infection and spreading of infection by P. aeruginosa. Therefore understanding of the role of pigments in biotechnology and its use in treatment diseases can be interesting. Methods: In this study, the pigment pyomelanin, pyoverdine, pyocyanin and pyorubrin P. aeruginosa that was produced in Tryptic Soy Broth (TSB),was separated with chloroform. Certain concentration of pigments (1mg/ml) were prepared and the antibacterial effect of all four pigment on Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) was determined by disk diffusion method. Results: The results showed that pyocyanin had antimicrobial effect on S. aureus but it had no effect on E. coli. while other pigments have no certain antimicrobial effect on this bacteria. Conclusions: Gram-positive bacteria (S. aureus), compared with Gram-negative bacteria (E. coli)were susceptible to Pyocyanin inhibitory effect. Generally, in future, these compounds (pyocyanin) can be used to treat diseases caused by S. aureus.
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