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Title
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Evaluation of the bacterial culture method in the diagnosis of mastitis compared to SCC and CMT
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Type
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Presentation
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Keywords
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Mastitis, bacterial culture, California mastitis test (CMT), Somatic cell count (SCC).
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Abstract
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Background and Aim:Different microbial agents can cause mastitis or inflammation of the mammary gland in cow. Considering health care and economic importance of bovine mastitis, evaluation of methods for detection of mastitis is very important. The aim of this study was to evaluate the microbial culture method for the diagnosis of mastitis compared to the somatic cell count (SCC) and California mastitis test (CMT) in some dairy herds in Hamedan province of Iran. Methods:Totally, 142 milk samples were collected from cattle suspected to mastitis from 6 dairy herds from September to November 2013. The sampling process was done under aseptic conditions. Immediately after transferring the samples to the laboratory, CMT and SCC assays were carried out on the samples and in order to identify the bacterial mastitis, the samples were also cultured on the Blood agar media. The gram staining method was performed on suspected colonies. Results:The results showed that about 90% of all cultured samples on blood agar contained a high number of suspected bacterial colonies. Whereas, 60% of the samples were only positive by SCC and CMT assays. The samples contained higher than 300,000 cell/ml were considered positive cases of bovine mastitis in SCC test. A score of 1 or more in CMT was also considered a positive result. Comparison of the results of SCC and CMT assays showed that SCC is a more precise method for diagnosis of bovine mastitis. The present experiment showed that this test was able to determine mastitic cases almost 10% more accurately than CMT test and as expected, CMT had lower sensitivity compared to the bacterial culture method. Conclusion:According to health care and economic impact of bovine mastitis, rapid and accurate diagnosis of this disease seems to be very important in dairy cattle industry. Regarding relative correlation between the results of SCC and CMT assays, these tests can be used for primitive diagnosis of bovine mastitis in dairy farms. However, the
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Researchers
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Abdolmajid Mohammadzadeh (Second Researcher), (Not In First Six Researchers), Pezhman Mahmoodi (Fifth Researcher), Ali Sadeghi-nasab (Fourth Researcher), Mohamadreza Pajohi-Alamoti (Third Researcher), (First Researcher)
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