|
Abstract
|
Current research is aimed at investigating micropropagation of GF677 (peach and almond hybrid) rootstock using multi-wall carbon nanotubes (MWCNTs). Single-node explants were cultured in WPM medium for shoot proliferation, containing BA (0, 4.4, 11 and 22 µM) and MWCNTs (0 and 10 mg/l); and then shoot number, shoot elongation, leaf area index, fresh and dry weight and dry mass were evaluated. Moreover, toxicity of MWCNTs was studied by measuring chlorophyll content and electrolyte leakage of grown buds. Results indicated positive effect of MWCNTs on shoot number and shoot elongation, and increased weight and leaf area. Compared to control, proliferation was found to be significantly higher for explants cultured on the medium containing MWCNTs together with low concentration of BA. Investigation of grown bud tissues by TEM imaging confirmed penetration of MWCNTs into the tissues. MWCNTs were found in bud and stem tissues affecting proliferation and growth rates. It seems that MWCNTs are able to create water pores in cell wall supporting water uptake. Furthermore, there were no significant differences between control and MWCNTs treated explants in chlorophyll content and electrolyte leakage, which could explain non-toxic effect of MWCNTs.
|