Background: Equine Granulocytic Anaplasmosis (EGA), a common, seasonal, non-contagious, ticktransmitted disease of equids observed where the Ixodes tick vector is founded. Horses less than four years of age have milder clinical signs and may present with only fever and mild depression in Iran. Methods: This study aimed to screen Equidae some regiones of Iran, for EGA. A total of 152 Equidae blood samples (equestrian horses = 100, farm horses = 18, and donkeys = 34) from Mazandaran (Amol and Mahmoodabad Hamedan , Amol, Hamadan (Malair and Hamadan), Arak (Komijan) and Lorestan (Borujerd and Rumshkan) were sampled from April 1401 to June 1402. Blood samples (up to 5 mL) were collected from the jugular vein into the labeled vacuum tubes containing EDTA for PCR analysis and kept at - 20 °C until testing. Giemsa-stained thin blood smears were made immediately after peripheral blood collection and further Results: No positive case was found in the microscopic examination while one case (0.65%) was reported positive by the nested-PCR method. Further studies should investigate the A. phagocytophilum occurring in other regions. Conclusion: Further studies should investigate the A. phagocytophilum occurring in other regions.
