Objective: The horses have been a symbol of bravery and power since ancient days and this taboo is still exists in our society. Equine acts as a main means of transport of goods and economy for poor farmers.This study aimed to determine the prevalence of gastro-intestinal parasites of horse in five Village ofHamedan province. Methods: Fecal samples for detection of gastrointestinal parasites were collected from 40 working horses ( 17 males and 23 females ) from May to June 2019 from 5 villages in Hamedan, West of Iran .The samples microscopically examined using techniques included: Clayton- laen method of floatation test using saturated sodium chloride solutions and sugar saturated solutions. Results: Fecal samples of 6 horses (15%) were negative for parasite eggs or oocysts. 57.5% were infected with a single parasite type and 10 (25 %) of horses had multiple infections with two and three parasites. The highest prevalence belonged to Parascaris( 32.5%). The overall prevalence of intestinal parasites eggs and oocyst in the positive horses were Parascaris equorum ( 32.5%), Dictyocaulus 10%, Dicrocoelium dendriticum 7.5%, Eimeria leuckarti 5%, Strongyle 2.5%. Conclusion: This study suggests that the high rate of infection with gastrointestinal parasites could contribute to low performance and life expectancy of working horses in the region. Keywords: Horse, Parasite, Hamedn
