In nanotechnology science several methods of chemical synthesis of silver nanoparticles (AgNps) are known. These methods involve high pressure, temperature and energy.Development in the green synthesis of Ag Nps is an important step in the field of application in nanotechnology. Accordingly, researchersare developing green methods that are eco-friendly, cost-effective and energy-efficient. Herein we report a green approach for the synthesis of AgNps. Thec olloidal silver particles were synthesized by reduction of AgNO with D (+) lactose as a reducing agent.The morphology and structure of synthesized Ag Nps were characterized usingRayleigh scattering, transmission electron microscopy (TEM), UV/Vis spectrophotometry, dynamic light scattering (DLS) and X-ray diffraction method (XRD). Also the Ag Nps in different concentration and shapes wereanalyzed for their antimicrobial activity agansit 4 positive and negative bacteriausing agar disc diffusion methodand fungusFusarium oxysporum cultivated on Potatoes Dextrose Agar medium (PDA). Results indicated that synthesized Ag Nps are relatively same in size (15 to 40 nm) and almost spherical.Also resultsshowed that the AgNps had inhibition activity against testedmicrobials, especially on Staphylococcus aureus (+), (C 27 3 :16 ± 0.55 mm)and Fusarium oxysporum (0 to 100%). The effects of concentration and size of the synthesized Ag NPs on antimicrobial activity were also discussed.
