Aim and Background: We report herein a flexible fluorescent nanofibrous membrane (FFNFM) prepared by incorporating the nitrogen and sulfur co doped carbon quantum dots (N, S-CDs) on electrospun polyvinyl alcohol nanofibrous membrane for sensitive detection of Doxorubicin (DOX) based on fluorescence resonance energy transfer (FRET). Doxorubicin is an antitumor agent which has a toxic effect on body tissues. Therefore, monitoring of the level of this drug in biological fluids of patients during their treatment period is important. Methods: N,S-CDs were synthesized by hydrothermal route from citric acid as the carbon source and rubeanic acid as the N, S source in the fashion of doping in order to improve the quantum yields of the carbon dots. After the treatment of PVA solution, various concentrations of N, S-carbon dots solution were added to the PVA solution. The processing parameters, including viscosity of PVA solution, feed rate, applied voltage and distance between the electrodes were optimized. Results and discussion: Under rthe optimum conditions, the linear range for the detection of DOX was 1.0 ×10-8 – 15.0 × 10-6 mol L-1 with a detection limit (S/N = 3) 54.0× 10-10 molL−1. The relative standard deviation for three replicate measurements of dOX was 1.7 %. A systematic comparison was performed between the sensing performance of FFNFM and N, S-CDs solution. Conclusion: A portable, sensitive, easy to-use, flexible and biocompatible FNFM for detection of Doxorubicin was developed a. To the best of our knowledge, efficient detection of DOX in biological samples based on such solid support has been reported for the first time.
