Introduction: Cerebrospinal fluid (CSF) is produced by the choroid plexuse into the brain.The embryonic cerebrospinal fluid (e-CSF) has been composed of various growth factors and morphogens factors. Adipose tissue derived stem cells (ADSCs) are multipotent stem cells wich were located in inguinal region of male Wistar rats. In this study, the effect of e-CSF on expression of early neural marker βIII-tubulin was investigated. Methods: In this study embryonic CSF was collected from Wistar Rat embryos at 17, 18 and 19 gestational ages. The ADSCs was isolated from inguinal region of male Wistar rats. ADSCs suspension cultured in the plate then, these cells were treated by E17, E18 and E19 e-CSF with 10% ratio (v/v). Results: The results showed that E17, E18 and E19 e-CSF causes considerable induction of neural-like morphological changes in ADSCs comparing with controls. The results show that eCSF, in addition to induction of significant morphological changes in ADSCs, increased the βIIItubulin expression comparing to the controls because of posing growth factors. Conclusion: The results of this study proved that e- CSF can induce neuronal differentiation in mesenchymal stem cell derived from Adipose tissue. Therefore, the data presented here support an important role of e-CSF for therapeutic use in neurodegenerative diseases.
