Objective: Cell migration plays key role in normal and pathologic condition of development of nervous system. Studies show that variety of signaling molecules (growth factors, cytokines, neurothrophines and extracellular matrix) regulate migratory behaviors of neural stem cells. Since, embryonic cerebrospinal fluid contains many signaling molecules. So, in this study we aimed to evaluate the effect of e-CSF on migration of neural stem cells. Materials and methods: e-CSF was collected from different days old (17, 18, 19 and 20) embryonic Wistar rat. neurosphere culture method was used for culturing neural stem cells obtained from embryonic subventricular zone (SVZ). Cultures were divided in two groups: 1. CSF treated group and 2. Control group (without e-CSF). morphometric characters were analyzed by image analyzer software (Image J). Protein expression was measured by immunocytochemistry. Results: Stemness characterization was assessed by neurosphere forming, nestin expression and serially passaging. Results show that e-CSF enhance chain migration of neurospheres. Chain migration enhanced in cultures treated with e-CSF from E17 and E18. Conclusion Neural stem cells migration have critical role in normal and abnormal brain development. Because e-CSF have an important role in migratory behavior of neural stem cells and, therefore, this fluid provide an alternative terapeutic for treatment of neural development disorders.
