سیامک یاری

Introduction:Neural tube in mammalians filled with cerebrospinal fluid and neuroepithelial cells is in close contact with this fluid.In adult ,cerebrospinal fluid involved in providing hydrostatic pressure and elimination of toxins .Recent years some evidence suggest developmental roles for embryonic cerebrospinal fluid .here we examined the effect of embryonic cerebrospinal fluid on embryonic ventricular zone derived neurosphere. Methods: in this study we used primary neurosphere culture, in brief cortex of 18 day old embryonic Wistar rat dissected out and enzymatically dissociated to single cells. Cell suspension seeded in DMEM/F12 medium supplemented with 1%N2, 1% antibiotic and 10 nano gram/ml FGF-2, 20 nano gram/ml EGF as control. CSF was collected from rat embryos then added in 10/100 (v/v) ratio to the medium as experimental group. Results:embryonic cerebrospinal fluid enhanced neurosphere growth in presence of epidermal growth factor and fibroblast growth factor-2.compared to treated neurospheres ,control neurospheres were smaller and exhibited redused prolifrerating cells. Conclusion:our data demonstrated that survival of neural progenitor cells enhanced by embryonic cerebrospinal fluid.