The purpose of this study was to investigate the relationship between university education and agricultural students' entrepreneurial spirit. To do this study, 150 samples were selected by using stratified random sampling methods among senior students both on graduated and undergraduates levels in Bu-Ali Sina University. Data collection tool was a researcher made questionnaire which its validity was verified by a group of experts and professors. The reliability of the questions was tested during a pre-test process using a Cronbach's alpha test and it was between 0.75 and 0.93. Descriptive results showed that entrepreneurial spirit of the students was between moderate to high and among entrepreneurial spirit indicators the amount of selfconfidence was highest but risk taking was the lowest. The results of Pearson test revealed that university education at each three level of knowledge and skill, socialcommunicational, and individual had a significant relationship with entrepreneurial spirit of the agricultural students.