With more than 70 species in Iran, Onobrychis miller is the biggest genus of the Fabaceae family and is mainly used as fodder due to its nutritional value. For the first time, in this paper, somatic embryogenesis and organogenesis processes are reported using different explants obtained from seedlings (hypocotyle, epicotyle, and cotyledon) of Onobrychis altissima species. Different cotyledon, hypocotyle, and epicotyle explants were cultured in the MS (Murashige and Skoog) medium using two concentrations of BAP (6-Benzylaminopurine) and 2, 4- Dichlorophenoxyacetic acid hormones. Frequency of callus induction and new organs were analyzed after two or three subcultures. The results were categorized using Duncan’s test. The maximal callus induction on different explants (hypocotyle, epicotyle, and cotyledon) was observed in concentrations of 1 mg and 5 mg per liter of 1-naphthaleneacetic acid (NAA) hormone and concentrations of 0.5 mg/liter and 5 mg/liter of BAP hormone. The largest root induction frequency was observed in MS planting medium having only NAA or low BAP concentrations and maximal germ induction frequency was reported in MS medium with high BAP concentrations compared to NAA treatment.
