Aim: The aim of this study was to evaluate Lotus corniculatus L. hydro-ethanolic extract healing effect on skin wound in male rat compared with phenytoin cream 1%, and complementary histological study on the healing process in the understudy groups. Method: After induction of anesthesia in 80 male Wistar rats, using 2*1shablon and Surgical blade a full-thickness rectangular wound with an area of 2cm2 was made behind their head and the skin was removed. Male Wistar rats were divided into 8 groups of ten including; the control (A), phenytoin 1% (B), Lotus corniculatus hydroethanolic extract 10% (C), Lotus corniculatus hydroethanolic extract 20% (D), Lotus corniculatus hydroethanolic extract 40% (E), phenytoin cream1% + Lotus corniculatus hydroethanolic extract 10% (F), phenytoin cream1% + Lotus corniculatus hydroethanolic extract 20% (G) and phenytoin cream 1% + Lotus corniculatus hydroethanolic extract 40% (H). Wound measurement with digital image analysis and medication prescription was performed for 21 days. In microscopic studies, biopsy was performed from wound healing site on the days 3,7,14, and 21. Considering histopathologic factors healing outcome for all groups were determined. The results were analyzed using SPSS 18 software. Results: The results showed that based on treatment period; histometric findings, the mean of the healed wound area in groups H (phenytoin cream 1% + Lotus corniculatus hydroethanolic extract) and E (Lotus corniculatus hydroethanolic extract 40%) was lower than other groups (p<0.01). Also based on the histopathological results, the outcome of healing in these groups was better than the other groups. Conclusion: Considering the anti-inflammatory, anti-microbial and healing effects of Lotus corniculatus hydroethanolic extract compared with phenytoin cream 1%, it can be claimed that its components are more effective in the healing of full-thickness skin wounds.
