STUDY OF PROTECTIVE EFFECT OF AVICENNIA MARINA HYDROETHANOLIC LEAF EXTRACT ON SPERMATOGENESIS IN MALE RAT INDUCED WITH CARBON TETRACHLORIDE Zohreh, Soleimani,* Mirazi, Naser Biology Department, Faculty of Basic Sciences, Bu-Ali Sina University, Hamadan, Iran Email:zohre.solymani@gmail.com The toxic chemical compounds are widelyused in the world. Carbon tetrachloride which is used in hygiene industries caused tissue disorders. Medicinal plants have protective effects in body tissues. In this study the protective effect of Avicennia marina leaf extract (MLE) on spermatogenesis in male rat were induced with ccl4 investigating.The 42 male rats with 220-250 gr body weight were divided randomly in 6 groups(n=7): control (taking normal saline,0.5ml/day, i,p ), sham(taking olive oil, 0.5ml/day, i,p single dose),group induced by ccl4(carbon tetrachloride 1:1 with olive oil,0.5ml single dose, i,p), treated groups: (1,2 and3 by carbon tetrachloride 1:1 with olive oil,0.5ml single dose and 200mg/Kg, 400mg/Kg and 800mg/kg MLE /day for 96 hrs, i,p). After the examination the blood samples were collected from heart directly and testosterone , FSH, LH , sperm count, sperm motility and GSI were analyzed and the microscopic studies of testes tissue were done. All data were expressed as mean±SEM. and statistical significance differences were accepted at P<0.05.Our results showed that the carbon tetrachloride has necrotic effect in testes. The testosterone, FSH and LH hormones increased in treated groups compared with ccl4 induced only group significantly (P<0.001). The number of sperm and motility were increased and microscopic study of testes tissue showed the necrosis and inflammation with decrease in spermatogonia and spermatocytes comparedwith ccl4 induced only group significantly (P<0.001) and treated groups were no changed. The Avicennia marina hydroethanolic extract has antioxidant and flavonoids compounds which can protect the testes tissues from toxic chemical agents.[1, 2, 3
