Cyclophosphamide is an anticancer drug which caused alkylation on DNA in cell. The side effect of cyclophosphamide are blood cells decrease and bone marrow damages. Hypericum perforatum is a medicinal plant which widely used in traditional medicine. In this study the hemopoeitic effect of Hypericumperforatumleaf extract (HPE) on bone marrow function and blood parameters in male rat were induced with cyclophosphamide was investigated. The 42 male rats with 220-250 gr body weight were divided randomly in 6 groups (n=7). control (taking normal saline,0.5ml/day, i,p ), group taking cyclophosphamide (15mg/kg/day, i.p.), group taking HPE (200mg/kg, i.p/day), treated groups: (1,2 and3 by cyclophosphamide ,15mg/kg/day, i.p. and 100mg/Kg, 200mg/Kg and 400mg/kg HPE /day for 10 days, i,p). After the examination the blood samples were collected from heart directly and RBC, WBC, Hct, Hb and PLT were analyzed and the microscopic studies of bone marrow tissue were done. All data were expressed as mean±SEM. and statistical significance differences were accepted at P<0.05. Our results showed that the cyclophosphamide has injury effect on bone marrow tissue. The blood cells count decreased in groups induced with cyclophosphamide significantly. The groups treated with HPE were significantly increased in blood cells and parameters compared with group taking cyclophosphamide (P<0.001). The microscopic study of stem cells showed that the basophilic erythroblast and band cells in increased cyclophosphamide induced group compared with HPE treated groups (P<0.01).The Hypericum perforatum hydoethanolic extract has antioxidant and flavonoids compounds which can protect the bone marrow tissues against toxic chemical drugs.
