نفیسه دعوتی

Introduction: Mostly research activities on domestic animals are concentrated on species and breeds of animals available in the temperate zones of Europe and North America. So the one humped camel (Camelus dromedarius) is certainly one of the most neglected species of the domestic animals (the subtropics zones ). Camel milk have unique nutrition and therapeutic properties. Such as other milk's domestic animals, lactic acid bacteria in Camel milk is domain microbial load. Objective: The aim of this study is investigation microbial load of camel milk and also isolation and identification of lactic acid bacteria for use in food industry. The first Bacterial isolates were identificated in genus level based on biochemical tests and then confirmed by molecular method. Materials & methods: Inoculum was done on PCA (48h, 37°C), YGC (3-5 days, 27°C) and MRS (24h, 37°C) for total count, total fungi count and LAB count respectively . Bacterial isolates were tested for gram reaction, catalase activity, cell morphology and biochemical properties to identification in genus level. To confirmation of LAB presence, amplification of 16S rRNA gene was carried out by universal primer. Results & discution: Log CFU for total count under anaerobic & aerobic condition, fungi and lactic acid bacteria count included 6.34 , 6.84, 3.41, 6.52 respectively. So share of LAB in microbial community is significant. 9 LAB was isolated. Based on results of biochemical tests, isolates of LAB belonged to lactobacillus and entrococcus genus. Presence of LAB was confirmed by band formation of amplicon in 1500 bp .The isolates of LAB are usful for dairy industry.